Bacteriophage Interference in Bacillus subtilis 168

Suppressor system in Bacillus subtilis 168.

Multiple auxotrophic strains of Bacillus subtilis 168 were tested for joint one-step reversion of two or more auxotrophic markers to the wild-type phenotype. Mu8u5u5, a strain requiring leucine, methionine, and threonine, yielded revertants that grew without added methionine or threonine and proved to have a suppressor gene. When transferred by transformation with deoxyribonucleic acid, this su...

Bacillus subtilis 168 4 5 6

Viral mutation affecting bacteriophage phi 1 development in Bacillus subtilis 168.

Bacillus subtilis bacteriophage phi 1m, a host-range variant, was isolated after mutagenesis of virulent bacteriophage phi 1. Unlike its wild-type antecedent, phi 1m could not form plaques on lawns of B subtilis 168 at 37 C, although it adsorbed to, penetrated, and killed this bacterium. Experiments conducted in liquid medium at 37 C showed that B. subtilis 168 cells allowed reduced levels of p...

Isolation of a Bacillus subtilis 168 derivative sensitive to defective bacteriophage PBSX.

A derivative of Bacillus subtilis strain 168 sensitive to defective bacteriophages PBSX and PBSZ has been isolated. This particular strain, RUB824, carries the genetic information for defective bacteriophage PBSX.

Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168.

A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to me...